Agilent Bio MAb
Agilent Bio MAb HPLC Columns
Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies.
Bio MAb columns are available in 1.7 µm, 3 µm, 5 µm, and 10 µm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column.
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- Features
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- A packing support composed of a rigid, spherical, highly cross-linked polystyrene divinylbenzene (PS/DVB) non-porous bead
- Particles grafted with a hydrophilic, polymeric layer, virtually eliminating non-specific binding of antibody proteins
- A different process is used to layer the weak cation-exchange phase to the particle making it a higher density than the Agilent Bio WCX column particles
- Specifically designed for the separation of charge isoforms of monoclonal antibodies
- Specifications
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Agilent Bio MAb Bonded Phase Weak Cation-Exchange (carboxylate) ID 2.1 and 4.6 mm Particle Size 1.7, 3, 5 and 10 µm pH Stability 2 - 12 Operating Temperature Limit 80 ºC Flow Rate 0.1 - 1 mL/min - Literature
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Agilent Bio MAb Data Sheet
Data sheet for the Agilent Bio MAb columns
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Agilent Bio Column Selection Guide
This comprehensive guide will help you find the right column for your characterization workflow. We have also included advice and tips on method development, solvent choice, mobile phase modification, optimization, and many example separations, all to assist you in column selection and method development.
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Agilent Bio MAb Application
Characterize Fab and Fc Fragments by Cation-exchange Chromatography. Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
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